Research

My research focuses on the development of molecular and nanostructured fluorescent probes based on different systems, which provide efficient fluorescent turn-on effects or spectral changes upon detection of cellular events (variation of pH, calcium concentration, reductive environment, ligand/receptor interaction, etc). I mainly developed calcium pH and plasma membrane probes but I am also involved in project aiming at developing nano-sensors based on nanoparticles and new functionalizable polymeric fluorescent nanoparticles for bio-imaging and theranostic.

Education/Career

I started studying biology and chemistry at the Université Pierre & Marie Curie (Paris VI) where I finally graduated in molecular chemistry. I started a PhD at the École Normale Supérieure (ENS) de Paris in 2004 on carbohydrate medicinal chemistry, more specifically in the synthesis of oligosaccharides. In 2008 I joined the Pr. Seeberger’s group as a postdoc (ETH Zürich and Max Planck Institute Berlin) where I worked on the automated synthesis of Glycocsaminoglycans and on multivalent carbohydrates systems. In 2010 I went back to Paris where I changed the topic of my research and where I started developing fluorescent probes and nanostructured sensors. In 2013, I obtained a CNRS researcher position in Strasbourg.

Selected Publications

Collot, M.; Ashokkumar, P.; Anton, H.; Boutant, E.; Faklaris, O.; Galli, T.; Mély, Y.; Danglot, L.; Klymchenko, A. S. MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience. Cell Chem. Biol. 2019. https://doi.org/10.1016/j.chembiol.2019.01.009.

Collot, M.; Bou, S.; Fam, T. K.; Richert, L.; Mély, Y.; Danglot, L.; Klymchenko, A. S. Probing Polarity and Heterogeneity of Lipid Droplets in Live Cells Using a Push–Pull Fluorophore. Anal. Chem. 2019, 91 (3), 1928–1935. https://doi.org/10.1021/acs.analchem.8b04218

Collot, M.; Boutant, E.; Lehmann, M.; Klymchenko, A. S. BODIPY with Tuned Amphiphilicity as a Fluorogenic Plasma Membrane Probe. Bioconjug. Chem. 2019, 30 (1), 192–199. https://doi.org/10.1021/acs.bioconjchem.8b00828

Collot, M.; Fam, T. K.; Ashokkumar, P.; Faklaris, O.; Galli, T.; Danglot, L.; Klymchenko, A. S. Ultrabright and Fluorogenic Probes for Multicolor Imaging and Tracking of Lipid Droplets in Cells and Tissues. J. Am. Chem. Soc. 2018, 140 (16), 5401–5411. https://doi.org/10.1021/jacs.7b12817

Ponsot, F.; Shen, W.; Ashokkumar, P.; Audinat, E.; Klymchenko, A. S.; Collot, M. PEGylated Red-Emitting Calcium Probe with Improved Sensing Properties for Neuroscience. ACS Sens. 2017, 2 (11), 1706–1712. https://doi.org/10.1021/acssensors.7b00665

Collot, M.; Ponsot, F.; Klymchenko, A. S. Ca-NIR: A Ratiometric near-Infrared Calcium Probe Based on a Dihydroxanthene-Hemicyanine Fluorophore. Chem. Commun. 2017, 53 (45), 6117–6120. https://doi.org/10.1039/C7CC02418E

Despras, G.; Zamaleeva, A. I.; Dardevet, L.; Tisseyre, C.; Magalhaes, J. G.; Garner, C.; Waard, M. D.; Amigorena, S.; Feltz, A.; Mallet, J.-M.; Collot, M. H-Rubies, a New Family of Red Emitting Fluorescent PH Sensors for Living Cells. Chem. Sci. 2015, 6 (10), 5928–5937. https://doi.org/10.1039/C5SC01113B